Activation of Dendritic Cells by the Novel Toll-Like Receptor 3 Agonist RGC100
Joint Authors
Naumann, Kai
Schmitz, Marc
Petzold, Christiane
Rohayem, Jacques
Schwarze, Anett
Wehner, Rebekka
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Toll-like receptor (TLR) 3 agonists emerged as attractive candidates for vaccination strategies against tumors and pathogens.
An important mechanism of action of such agonists is based on the activation of TLR3-expressing dendritic cells (DCs), which display a unique capacity to induce and stimulate T-cell responses.
In this context, it has been demonstrated that targeting of TLR3 by double-stranded RNA such as poly(I:C) results in potent activation of DCs.
Major disadvantages of poly(I:C) comprise its undefined chemical structure and very poor homogeneity, with subsequent unpredictable pharmacokinetics and high toxicity.
In the present study, we evaluated the physicochemical properties and biological activity of the novel TLR3 agonist RGC100.
RGC100 has a defined chemical structure, with a defined length (100 bp) and molecular weight (64.9 KDa) and a good solubility.
RGC100 is stable in serum and activates myeloid DCs through TLR3 targeting, as evidenced by gene silencing experiments.
Activation of mouse and human myeloid CD1c+ DCs by RGC100 leads to secretion of several proinflammatory cytokines.
In addition, RGC100 improves the ability of CD1c+ DCs to stimulate T-cell proliferation.
Due to its physicochemical properties and its immunostimulatory properties, RGC100 may represent a promising adjuvant for prophylactic and therapeutic vaccination strategies.
American Psychological Association (APA)
Naumann, Kai& Wehner, Rebekka& Schwarze, Anett& Petzold, Christiane& Schmitz, Marc& Rohayem, Jacques. 2013. Activation of Dendritic Cells by the Novel Toll-Like Receptor 3 Agonist RGC100. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-460208
Modern Language Association (MLA)
Naumann, Kai…[et al.]. Activation of Dendritic Cells by the Novel Toll-Like Receptor 3 Agonist RGC100. Clinical and Developmental Immunology No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-460208
American Medical Association (AMA)
Naumann, Kai& Wehner, Rebekka& Schwarze, Anett& Petzold, Christiane& Schmitz, Marc& Rohayem, Jacques. Activation of Dendritic Cells by the Novel Toll-Like Receptor 3 Agonist RGC100. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-460208
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460208