Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells
Joint Authors
Wu, Mei-Chen
Hsiao, Nai-Wan
Yang, Jai-Sing
Wu, Shih-Hsiung
Lu, Tzu-Li
Chuang, Jing-Yuan
Hsu, Ching-Hsiang
Chiu, Shau-Ting
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Type-2 ribosome-inactivating proteins, composed of a toxic A-chain and lectin-like B-chain, display various biological functions, including cytotoxicity and immunomodulation.
We here cloned the lectin-like B-chain encoding fragment of a newly identified type-2 RIP gene, articulatin gene, from Viscum articulatum, into a bacterial expression vector to obtain nonglycosylated recombinant protein expressed in inclusion bodies.
After purification and protein refolding, soluble refolded recombinant articulatin B-chain (rATB) showed lectin activity specific toward galactoside moiety and was stably maintained while stored in low ionic strength solution.
Despite lacking glycosylation, rATB actively bound leukocytes with preferential binding to monocytes and in vitro stimulated PBMCs to release cytokines without obvious cytotoxicity.
These results implicated such a B-chain fragment as a potential immunomodulator.
American Psychological Association (APA)
Lu, Tzu-Li& Chuang, Jing-Yuan& Yang, Jai-Sing& Chiu, Shau-Ting& Hsiao, Nai-Wan& Wu, Mei-Chen…[et al.]. 2011. Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-460219
Modern Language Association (MLA)
Lu, Tzu-Li…[et al.]. Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-460219
American Medical Association (AMA)
Lu, Tzu-Li& Chuang, Jing-Yuan& Yang, Jai-Sing& Chiu, Shau-Ting& Hsiao, Nai-Wan& Wu, Mei-Chen…[et al.]. Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-460219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460219