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Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi : Morphometrical and Molecular Analysis of Intermediate Developmental Forms
Joint Authors
Manning-Cela, Rebeca G.
Hernández-Osorio, L. A.
Ballesteros-Rodea, G.
Márquez-Dueñas, C.
Florencio-Martínez, Luis E.
Martínez-Calvillo, Santiago
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Trypanosoma cruzi undergoes a biphasic life cycle that consists of four alternate developmental stages.
In vitro conditions to obtain a synchronic transformation and efficient rates of pure intermediate forms (IFs), which are indispensable for further biochemical, biological, and molecular studies, have not been reported.
In the present study, we established an improved method to obtain IFs from secondary amastigogenesis.
During the transformation kinetics, we observed progressive decreases in the size of the parasite body, undulating membrane and flagellum that were concomitant with nucleus remodeling and kinetoplast displacement.
In addition, a gradual reduction in parasite movement and acquisition of the amastigote-specific Ssp4 antigen were observed.
Therefore, our results showed that the in vitro conditions used obtained large quantities of highly synchronous and pure IFs that were clearly distinguished by morphometrical and molecular analyses.
Obtaining these IFs represents the first step towards an understanding of the molecular mechanisms involved in amastigogenesis.
American Psychological Association (APA)
Hernández-Osorio, L. A.& Márquez-Dueñas, C.& Florencio-Martínez, Luis E.& Ballesteros-Rodea, G.& Martínez-Calvillo, Santiago& Manning-Cela, Rebeca G.. 2009. Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi : Morphometrical and Molecular Analysis of Intermediate Developmental Forms. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-460226
Modern Language Association (MLA)
Hernández-Osorio, L. A.…[et al.]. Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi : Morphometrical and Molecular Analysis of Intermediate Developmental Forms. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-460226
American Medical Association (AMA)
Hernández-Osorio, L. A.& Márquez-Dueñas, C.& Florencio-Martínez, Luis E.& Ballesteros-Rodea, G.& Martínez-Calvillo, Santiago& Manning-Cela, Rebeca G.. Improved Method for In Vitro Secondary Amastigogenesis of Trypanosoma cruzi : Morphometrical and Molecular Analysis of Intermediate Developmental Forms. Journal of Biomedicine and Biotechnology. 2009. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-460226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460226