![](/images/graphics-bg.png)
ASGR1 and ASGR2, the Genes that Encode the Asialoglycoprotein Receptor (Ashwell Receptor), Are Expressed in Peripheral Blood Monocytes and Show Interindividual Differences in Transcript Profile
Joint Authors
Harris, Rebecca Louise
Bowen, Derrick John
van den Berg, Carmen Wilma
Source
Molecular Biology International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Background.
The asialoglycoprotein receptor (ASGPR) is a hepatic receptor that mediates removal of potentially hazardous glycoconjugates from blood in health and disease.
The receptor comprises two proteins, asialoglycoprotein receptor 1 and 2 (ASGR1 and ASGR2), encoded by the genes ASGR1 and ASGR2.
Design and Methods.
Using reverse transcription amplification (RT-PCR), expression of ASGR1 and ASGR2 was investigated in human peripheral blood monocytes.
Results.
Monocytes were found to express ASGR1 and ASGR2 transcripts.
Correctly spliced transcript variants encoding different isoforms of ASGR1 and ASGR2 were present in monocytes.
The profile of transcript variants from both ASGR1 and ASGR2 differed among individuals.
Transcript expression levels were compared with the hepatocyte cell line HepG2 which produces high levels of ASGPR.
Monocyte transcripts were 4 to 6 orders of magnitude less than in HepG2 but nonetheless readily detectable using standard RT-PCR.
The monocyte cell line THP1 gave similar results to monocytes harvested from peripheral blood, indicating it may provide a suitable model system for studying ASGPR function in this cell type.
Conclusions.
Monocytes transcribe and correctly process transcripts encoding the constituent proteins of the ASGPR.
Monocytes may therefore represent a mobile pool of the receptor, capable of reaching sites remote from the liver.
American Psychological Association (APA)
Harris, Rebecca Louise& van den Berg, Carmen Wilma& Bowen, Derrick John. 2012. ASGR1 and ASGR2, the Genes that Encode the Asialoglycoprotein Receptor (Ashwell Receptor), Are Expressed in Peripheral Blood Monocytes and Show Interindividual Differences in Transcript Profile. Molecular Biology International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-460240
Modern Language Association (MLA)
Harris, Rebecca Louise…[et al.]. ASGR1 and ASGR2, the Genes that Encode the Asialoglycoprotein Receptor (Ashwell Receptor), Are Expressed in Peripheral Blood Monocytes and Show Interindividual Differences in Transcript Profile. Molecular Biology International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-460240
American Medical Association (AMA)
Harris, Rebecca Louise& van den Berg, Carmen Wilma& Bowen, Derrick John. ASGR1 and ASGR2, the Genes that Encode the Asialoglycoprotein Receptor (Ashwell Receptor), Are Expressed in Peripheral Blood Monocytes and Show Interindividual Differences in Transcript Profile. Molecular Biology International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-460240
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460240