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Differential between Protein and mRNA Expression of CCR7 and SSTR5 Receptors in Crohn's Disease Patients
Joint Authors
Muller, Christian D.
Dumont, Serge
Vonesch, Jean-Luc
Taquet, Nathalie
Hentsch, Didier
Reimund, Jean-Marie
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Crohn's disease (CD) is a multifactorial chronic inflammatory bowel disease of unknown cause.
The aim of the present study was to explore if mRNA over-expression of SSTR5 and CCR7 found in CD patients could be correlated to respective protein expression.
When compared to healthy donors, SSTR5 was over-expressed 417 ± 71 times in CD peripheral blood mononuclear cells (PBMCs).
Flow cytometry experiments showed no correlation between mRNA and protein expression for SSTR5 in PBMCs.
In an attempt to find a reason of such a high mRNA expression, SSTR5 present on CD PBMCs were tested and found as biologically active as on healthy cells.
In biopsies of CD intestinal tissue, SSTR5 was not over-expressed but CCR7, unchanged in PBMCs, was over-expressed by 10 ± 3 times in the lamina propria.
Confocal microscopy showed a good correlation of CCR7 mRNA and protein expression in CD intestinal biopsies.
Our data emphasize flow and image cytometry as impossible to circumvent in complement to molecular biology so to avoid false interpretation on receptor expressions.
Once confirmed by further large-scale studies, our preliminary results suggest a role for SSTR5 and CCR7 in CD pathogenesis.
American Psychological Association (APA)
Taquet, Nathalie& Dumont, Serge& Vonesch, Jean-Luc& Hentsch, Didier& Reimund, Jean-Marie& Muller, Christian D.. 2010. Differential between Protein and mRNA Expression of CCR7 and SSTR5 Receptors in Crohn's Disease Patients. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-460409
Modern Language Association (MLA)
Taquet, Nathalie…[et al.]. Differential between Protein and mRNA Expression of CCR7 and SSTR5 Receptors in Crohn's Disease Patients. Mediators of Inflammation No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-460409
American Medical Association (AMA)
Taquet, Nathalie& Dumont, Serge& Vonesch, Jean-Luc& Hentsch, Didier& Reimund, Jean-Marie& Muller, Christian D.. Differential between Protein and mRNA Expression of CCR7 and SSTR5 Receptors in Crohn's Disease Patients. Mediators of Inflammation. 2010. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-460409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460409