Comparative Serological Assays for the Study of H5 and H7 Avian Influenza Viruses
Joint Authors
Milani, Adelaide
Molesti, Eleonora
Terregino, Calogero
Temperton, Nigel J.
Cattoli, Giovanni
Source
Influenza Research and Treatment
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The nature of influenza virus to randomly mutate and evolve into new types is an important challenge in the control of influenza infection.
It is necessary to monitor virus evolution for a better understanding of the pandemic risk posed by certain variants as evidenced by the highly pathogenic avian influenza (HPAI) viruses.
This has been clearly recognized in Egypt following the notification of the first HPAI H5N1 outbreak.
The continuous circulation of the virus and the mass vaccination programme undertaken in poultry have resulted in a progressive genetic evolution and a significant antigenic drift near the major antigenic sites.
In order to establish if vaccination is sufficient to provide significant intra- and interclade cross-protection, lentiviral pseudotypes derived from H5N1 HPAI viruses (A/Vietnam/1194/04, A/chicken/Egypt-1709-01/2007) and an antigenic drift variant (A/chicken/Egypt-1709-06-2008) were constructed and used in pseudotype-based neutralization assays (pp-NT).
pp-NT data obtained was confirmed and correlated with HI and MN assays.
A panel of pseudotypes belonging to influenza Groups 1 and 2, with a combination of reporter systems, was also employed for testing avian sera in order to support further application of pp-NT as an alternative valid assay that can improve avian vaccination efficacy testing, vaccine virus selection, and the reliability of reference sera.
American Psychological Association (APA)
Molesti, Eleonora& Milani, Adelaide& Terregino, Calogero& Cattoli, Giovanni& Temperton, Nigel J.. 2013. Comparative Serological Assays for the Study of H5 and H7 Avian Influenza Viruses. Influenza Research and Treatment،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-460448
Modern Language Association (MLA)
Molesti, Eleonora…[et al.]. Comparative Serological Assays for the Study of H5 and H7 Avian Influenza Viruses. Influenza Research and Treatment No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-460448
American Medical Association (AMA)
Molesti, Eleonora& Milani, Adelaide& Terregino, Calogero& Cattoli, Giovanni& Temperton, Nigel J.. Comparative Serological Assays for the Study of H5 and H7 Avian Influenza Viruses. Influenza Research and Treatment. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-460448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460448