Effects of Saponins against Clinical E. coli Strains and Eukaryotic Cell Line
Joint Authors
Kaca, Wiesław
Czerwonka, Grzegorz
Węgierek-Ciuk, Aneta
Arabski, Michał
Lankoff, Anna
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Saponins are detergent-like substances showing antibacterial as well as anticancer potential.
In this study, the effects of saponins from Quillaja saponaria were analyzed against prokaryotic and eukaryotic cells.
Multidrug-resistant clinical E.
coli strains were isolated from human urine.
As eukaryotic cells, the CHO-K1 cell lines were applied.
Antibacterial effect of ampicillin, streptomycin, and ciprofloxacin in the presence of saponins was measured by cultivation methods.
Properties of saponins against CHO-K1 cells were measured by the MTT test, hemolysis assay and flow cytometry.
Saponin from Quillaja saponaria has a cytotoxic effect at concentrations higher than 25 μg/mL and in the range of 12–50 μg/mL significantly increases the level of early apoptotic cells.
Saponin at dose of 12 μg/mL enhances the six E.
coli strains growth.
We postulate that saponins increase the influx of nutrients from the medium into E.
coli cells.
Saponins do not have synergetic effects on antibacterial action of tested antibiotics.
In contrary, in the presence of saponins and antibiotics, more CFU/mL E.
coli cells were observed.
This effect was similar to saponins action alone towards E.
coli cells.
In conclusion, saponins was cytotoxic against CHO-K1 cells, whereas against E.
coli cells this effect was not observed.
American Psychological Association (APA)
Arabski, Michał& Węgierek-Ciuk, Aneta& Czerwonka, Grzegorz& Lankoff, Anna& Kaca, Wiesław. 2012. Effects of Saponins against Clinical E. coli Strains and Eukaryotic Cell Line. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-460457
Modern Language Association (MLA)
Arabski, Michał…[et al.]. Effects of Saponins against Clinical E. coli Strains and Eukaryotic Cell Line. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-460457
American Medical Association (AMA)
Arabski, Michał& Węgierek-Ciuk, Aneta& Czerwonka, Grzegorz& Lankoff, Anna& Kaca, Wiesław. Effects of Saponins against Clinical E. coli Strains and Eukaryotic Cell Line. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-460457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460457