An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues
Joint Authors
Silva-Pereira, Ildinete
Duarte, Gilvan Caetano
Rubini, Marciano Regis
Kyaw, Cynthia Maria
Mello-de-Sousa, Thiago Machado
Ferreira Filho, Edivaldo Ximenes
Poças-Fonseca, Marcio Jose
de Faria, Fabrícia Paula
Tavares, Eveline Queiroz de Pinho
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes.
This work describes the A.
nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme.
Active recombinant endoglucanase A (rEG A) was efficiently secreted as a 35 kDa protein which was purified through a two-step chromatography procedure.
The highest enzyme activity was detected at 50°C/pH 4.
rEG A retained 100% of activity when incubated at 45 and 55°C for 72 h.
Purified rEG A kinetic parameters towards CMC were determined as Km=27.5±4.33 mg/mL, Vmax=1.185±0.11 mmol/min, and 55.8 IU (international units)/mg specific activity.
Recombinant P.
pastoris supernatant presented hydrolytic activity towards lignocellulosic residues such as banana stalk, sugarcane bagasse, soybean residues, and corn straw.
These data indicate that rEG A is suitable for plant biomass conversion into products of commercial importance, such as second-generation fuel ethanol.
American Psychological Association (APA)
Tavares, Eveline Queiroz de Pinho& Rubini, Marciano Regis& Mello-de-Sousa, Thiago Machado& Duarte, Gilvan Caetano& de Faria, Fabrícia Paula& Ferreira Filho, Edivaldo Ximenes…[et al.]. 2013. An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues. Enzyme Research،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-460556
Modern Language Association (MLA)
Tavares, Eveline Queiroz de Pinho…[et al.]. An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues. Enzyme Research No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-460556
American Medical Association (AMA)
Tavares, Eveline Queiroz de Pinho& Rubini, Marciano Regis& Mello-de-Sousa, Thiago Machado& Duarte, Gilvan Caetano& de Faria, Fabrícia Paula& Ferreira Filho, Edivaldo Ximenes…[et al.]. An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-460556
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460556