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The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts
Joint Authors
Golding, Sarah E.
Rosenberg, Elizabeth
Dever, Seth M.
Lin, Peck Sun
Jameson, Mark J.
Broaddus, William C.
Chung, Theodore D.
Valerie, Kristoffer
Khalil, Ashraf A.
Source
International Journal of Molecular Imaging
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Bioluminescence imaging (BLI) is a relatively new noninvasive technology used for quantitative assessment of tumor growth and therapeutic effect in living animal models.
BLI involves the generation of light by luciferase-expressing cells following administration of the substrate luciferin in the presence of oxygen and ATP.
In the present study, the effects of hypoxia, hypoperfusion, and pH on BLI signal (BLS) intensity were evaluated in vitro using cultured cells and in vivo using a xenograft model in nude mice.
The intensity of the BLS was significantly reduced in the presence of acute and chronic hypoxia.
Changes in cell density, viability, and pH also affected BLS.
Although BLI is a convenient non-invasive tool for tumor assessment, these factors should be considered when interpreting BLS intensity, especially in solid tumors that could be hypoxic due to rapid growth, inadequate blood supply, and/or treatment.
American Psychological Association (APA)
Khalil, Ashraf A.& Jameson, Mark J.& Broaddus, William C.& Lin, Peck Sun& Dever, Seth M.& Golding, Sarah E.…[et al.]. 2013. The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts. International Journal of Molecular Imaging،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-460596
Modern Language Association (MLA)
Khalil, Ashraf A.…[et al.]. The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts. International Journal of Molecular Imaging No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-460596
American Medical Association (AMA)
Khalil, Ashraf A.& Jameson, Mark J.& Broaddus, William C.& Lin, Peck Sun& Dever, Seth M.& Golding, Sarah E.…[et al.]. The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts. International Journal of Molecular Imaging. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-460596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460596