Contribution of Ca2+-Dependent Cl− Channels to Norepinephrine-Induced Contraction of Femoral Artery Is Replaced by Increasing EDCF Contribution during Ageing
Joint Authors
Behuliak, Michal
Petrova, Miriam
Zicha, Josef
Liskova, Silvia
Karen, Petr
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The activation of Ca2+-dependent Cl− channels during norepinephrine-induced contraction of vascular smooth muscle was suggested to depolarize cell membrane and to increase Ca2+ entry.
Hypertension and ageing are associated with altered Ca2+ handling including possible activation of Ca2+-dependent Cl− channels.
Our study was aimed to determine Ca2+-dependent Cl− channels contribution to norepinephrine-induced contraction during hypertension and ageing.
Norepinephrine-induced concentration-response curves of femoral arteries from 6- and 12-month-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were recorded using wire myograph.
Pretreatment with Ca2+-dependent Cl- channel inhibitor indanyloxyacetic acid 94 [R(+)-IAA-94](IAA) attenuated norepinephrine-induced contraction in all groups, but relatively more in WKY than SHR arteries.
The attenuation of norepinephrine-induced contraction after Ca2+-dependent Cl− channels blockade was partially reduced in 12-month-old WKY rats, but substantially diminished in 12-month-old SHR.
IAA effect was enhanced after NO synthase inhibition but decreased by ageing.
In 20-month-old WKY rats norepinephrine-induced contraction was not affected by IAA but was almost abolished after cyclooxygenase inhibition by indomethacin or niflumic acid.
In conclusion, contribution of Ca2+-dependent Cl− channels to norepinephrine-induced contraction diminished with age, hypertension development, and/or NO synthesis inhibition.
Ca2+-dependent Cl− channels are important for maintenance of normal vascular tone while their inactivation/closing might be a pathological mechanism.
American Psychological Association (APA)
Liskova, Silvia& Petrova, Miriam& Karen, Petr& Behuliak, Michal& Zicha, Josef. 2014. Contribution of Ca2+-Dependent Cl− Channels to Norepinephrine-Induced Contraction of Femoral Artery Is Replaced by Increasing EDCF Contribution during Ageing. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-460652
Modern Language Association (MLA)
Liskova, Silvia…[et al.]. Contribution of Ca2+-Dependent Cl− Channels to Norepinephrine-Induced Contraction of Femoral Artery Is Replaced by Increasing EDCF Contribution during Ageing. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-460652
American Medical Association (AMA)
Liskova, Silvia& Petrova, Miriam& Karen, Petr& Behuliak, Michal& Zicha, Josef. Contribution of Ca2+-Dependent Cl− Channels to Norepinephrine-Induced Contraction of Femoral Artery Is Replaced by Increasing EDCF Contribution during Ageing. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-460652
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460652