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Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture
Joint Authors
Yan, Zheng-Jie
Liu, Jia-Yin
Cui, Yu-Gui
Shi, Zhan-Ping
Xue, Zhi-Gang
Dong, Juan
Chen, Juan
Qian, Xiao-Qiao
Liao, Ting-Ting
Fan, Guoping
Sun, Xue-Ping
Wang, Yan-Peng
Source
International Journal of Cell Biology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-13
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Human primary fibroblasts are a popular type of somatic cells for the production of induced pluripotent stem (iPS) cells.
Here we characterized biological properties of primary fibroblasts in terms of cell-growth rate, cytogenetic stability, and the number of inactive X chromosomes during long-term passaging.
We produced eight lines of female human dermal fibroblasts (HDFs) and found normal karyotype and expected pattern of X chromosome inactivation (XCI) at low passages (Passage P1-5).
However, four out of the eight HDF lines at high passage numbers (≥P10) exhibited duplicated hallmarks of inactive X chromosome including two punctuate signals of histone H3 lysine 27 trimethylation (H3K27me3) and X inactive-specific transcript (XIST) RNA signals in approximately 8.5–18.5% of the cells.
Our data suggest that the copy number of inactive X chromosomes in a subset of female HDF is increased by a two-fold.
Consistently, DNA fluorescent in situ hybridization (FISH) identified 3-4 copies of X chromosomes in one nucleus in this subset of cells with two inactive Xs.
We conclude that female HDF cultures exhibit a higher risk of genetic anomalies such as carrying an increased number of X chromosomes including both active and inactive X chromosomes at a high passage (≥P10).
American Psychological Association (APA)
Xue, Zhi-Gang& Shi, Zhan-Ping& Dong, Juan& Liao, Ting-Ting& Wang, Yan-Peng& Sun, Xue-Ping…[et al.]. 2011. Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture. International Journal of Cell Biology،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-460684
Modern Language Association (MLA)
Xue, Zhi-Gang…[et al.]. Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture. International Journal of Cell Biology No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-460684
American Medical Association (AMA)
Xue, Zhi-Gang& Shi, Zhan-Ping& Dong, Juan& Liao, Ting-Ting& Wang, Yan-Peng& Sun, Xue-Ping…[et al.]. Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture. International Journal of Cell Biology. 2011. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-460684
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460684