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Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells
Joint Authors
Langsner, Robert J.
Chang, Joseph
Bickford, Lissett R.
Drezek, Rebekah
Agollah, Germaine D.
Kennedy, Laura C.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Tumor margin detection for patients undergoing breast conservation surgery primarily occurs postoperatively.
Previously, we demonstrated that gold nanoshells rapidly enhance contrast of HER2 overexpression in ex vivo tissue sections.
Our ultimate objective, however, is to discern HER2 overexpressing tissue from normal tissue in whole, nonsectioned, specimens to facilitate rapid diagnoses.
Here, we use targeted nanoshells to quickly and effectively visualize HER2 receptor expression in intact ex vivo human breast tissue specimens.
Punch biopsies of human breast tissue were analyzed after a brief 5-minute incubation with and without HER2-targeted silica-gold nanoshells using two-photon microscopy and stereomicroscopy.
Labeling was subsequently verified using reflectance confocal microscopy, darkfield hyperspectral imaging, and immunohistochemistry to confirm levels of HER2 expression.
Our results suggest that anti-HER2 nanoshells used in tandem with a near-infrared reflectance confocal microscope and a standard stereomicroscope may potentially be used to discern HER2-overexpressing cancerous tissue from normal tissue in near real time and offer a rapid supplement to current diagnostic techniques.
American Psychological Association (APA)
Bickford, Lissett R.& Langsner, Robert J.& Chang, Joseph& Kennedy, Laura C.& Agollah, Germaine D.& Drezek, Rebekah. 2012. Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells. Journal of Oncology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-460925
Modern Language Association (MLA)
Bickford, Lissett R.…[et al.]. Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells. Journal of Oncology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-460925
American Medical Association (AMA)
Bickford, Lissett R.& Langsner, Robert J.& Chang, Joseph& Kennedy, Laura C.& Agollah, Germaine D.& Drezek, Rebekah. Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-460925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460925