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An IMSATP Assay for the Detection of Mycobacterium tuberculosis in Urine
Joint Authors
Source
Tuberculosis Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Although sputum smears are the gold standard for diagnosis of tuberculosis, sensitivity in HIV/TB coinfection cases is low, indicating a need for alternative methods.
Urine is being increasingly evaluated.
Materials and Methods.
A novel method for detecting Mycobacterium tuberculosis (MTB) in synthetic urine using a combined IMS/ATP assay was evaluated.
Preliminary work established standard ATP conditions and the sensitivity and specificity of the MTB antibody.
Eighty-four blinded samples in four replicate assays were evaluated for the presence of MTB using labeled immunomagnetic beads for capture.
Beads were separated, washed, and resuspended in broth and added to a microtiter plate.
Bioluminescent output was measured and signal-to-noise ratios were calculated.
All samples were plated on Middlebrook 7H10 agar or trypticase soy agar to determine limit of detection and recoveries.
Results and Conclusions.
MTB was distinguished from common bacteriuria isolates and other nontarget bacteria by its ATP results.
IMS/ATP successfully detected 19 of 28 samples of MTB in synthetic urine with a limit of detection of 104 CFU/ml.
Sensitivity and specificity were 67.9% and 82.1%, respectively.
This assay offers a possible rapid screening method for HIV-positive patients with suspected coinfection to improve MTB diagnosis.
American Psychological Association (APA)
Hunter, Dawn M.& Lim, Daniel V.. 2012. An IMSATP Assay for the Detection of Mycobacterium tuberculosis in Urine. Tuberculosis Research and Treatment،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-460979
Modern Language Association (MLA)
Hunter, Dawn M.& Lim, Daniel V.. An IMSATP Assay for the Detection of Mycobacterium tuberculosis in Urine. Tuberculosis Research and Treatment No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-460979
American Medical Association (AMA)
Hunter, Dawn M.& Lim, Daniel V.. An IMSATP Assay for the Detection of Mycobacterium tuberculosis in Urine. Tuberculosis Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-460979
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460979