Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells

Joint Authors

Yau Hsiung, Wong
Abdul Kadir, Habsah

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-03-01

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Medicine

Abstract EN

The anticancer potential of Leea indica, a Chinese medicinal plant was investigated for the first time.

The crude ethanol extract and fractions (ethyl acetate, hexane, and water) of Leea indica were evaluated their cytotoxicity on various cell lines (Ca Ski, MCF 7, MDA-MB-435, KB, HEP G2, WRL 68, and Vero) by MTT assay.

Leea indica ethyl acetate fraction (LIEAF) was found showing the greatest cytotoxic effect against Ca Ski cervical cancer cells.

Typical apoptotic morphological changes such as DNA fragmentation and chromatin condensation were observed in LIEAF-treated cells.

Early signs of apoptosis such as externalization of phosphatidylserine and disruption of mitochondrial membrane potential indicated apoptosis induction.

This was further substantiated by dose- and time-dependent accumulation of sub-G1 cells, depletion of intracellular glutathione, and activation of caspase-3.

In conclusion, these results suggested that LIEAF inhibited cervical cancer cells growth by inducing apoptosis and could be developed as potential anticancer drugs.

American Psychological Association (APA)

Yau Hsiung, Wong& Abdul Kadir, Habsah. 2011. Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-461008

Modern Language Association (MLA)

Yau Hsiung, Wong& Abdul Kadir, Habsah. Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-461008

American Medical Association (AMA)

Yau Hsiung, Wong& Abdul Kadir, Habsah. Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-461008

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-461008