Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells
Joint Authors
Yau Hsiung, Wong
Abdul Kadir, Habsah
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The anticancer potential of Leea indica, a Chinese medicinal plant was investigated for the first time.
The crude ethanol extract and fractions (ethyl acetate, hexane, and water) of Leea indica were evaluated their cytotoxicity on various cell lines (Ca Ski, MCF 7, MDA-MB-435, KB, HEP G2, WRL 68, and Vero) by MTT assay.
Leea indica ethyl acetate fraction (LIEAF) was found showing the greatest cytotoxic effect against Ca Ski cervical cancer cells.
Typical apoptotic morphological changes such as DNA fragmentation and chromatin condensation were observed in LIEAF-treated cells.
Early signs of apoptosis such as externalization of phosphatidylserine and disruption of mitochondrial membrane potential indicated apoptosis induction.
This was further substantiated by dose- and time-dependent accumulation of sub-G1 cells, depletion of intracellular glutathione, and activation of caspase-3.
In conclusion, these results suggested that LIEAF inhibited cervical cancer cells growth by inducing apoptosis and could be developed as potential anticancer drugs.
American Psychological Association (APA)
Yau Hsiung, Wong& Abdul Kadir, Habsah. 2011. Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-461008
Modern Language Association (MLA)
Yau Hsiung, Wong& Abdul Kadir, Habsah. Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-461008
American Medical Association (AMA)
Yau Hsiung, Wong& Abdul Kadir, Habsah. Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-461008
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461008
