Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3
Joint Authors
Ganguly, Sudershan
Chaudhuri, Surabhi
Nain, Lata
Dutta, Debjani
Pranaw, Kumar
Singh, Surender
Source
Biotechnology Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Information Technology and Computer Science
Abstract EN
Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum.
Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using response surface methodology (RSM).
The effects of all five chemical components (considered as independent variables), namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable) were studied, and it was found that tryptone and dextrose had maximum influence on protease production.
The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone—5.71, soya peptone—4.9, dextrose—1.45, NaCl—6.08, and dipotassium phosphate—0.47 in g/L).
To best of knowledge, there are no reports on optimization of medium component for protease production by X.
indica KB-3 using RSM and their application in fibrinolysis.
This study will be useful for industrial processes for production of protease enzyme from X.
indica KB-3 for its application in the field of agriculture and medicine.
American Psychological Association (APA)
Pranaw, Kumar& Singh, Surender& Dutta, Debjani& Chaudhuri, Surabhi& Ganguly, Sudershan& Nain, Lata. 2014. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. Biotechnology Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-461031
Modern Language Association (MLA)
Pranaw, Kumar…[et al.]. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. Biotechnology Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-461031
American Medical Association (AMA)
Pranaw, Kumar& Singh, Surender& Dutta, Debjani& Chaudhuri, Surabhi& Ganguly, Sudershan& Nain, Lata. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. Biotechnology Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-461031
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461031