![](/images/graphics-bg.png)
Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
Joint Authors
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide.
Commercially available premade phage libraries allow us to take screening in the easiest way.
On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions.
This paper focuses on what should be born in mind for beginners using commercially available cloning kits (Ph.D.
with type 3 vector and T7Select systems for M13 and T7 phage, respectively).
In the M13 system, Pro or a basic amino acid (especially, Arg) should be avoided at the N-terminus of peptide fused to gp3.
In both systems, peptides containing odd number(s) of Cys should be designed with caution.
Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.
American Psychological Association (APA)
Fukunaga, Keisuke& Taki, Masumi. 2012. Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-461255
Modern Language Association (MLA)
Fukunaga, Keisuke& Taki, Masumi. Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems. Journal of Nucleic Acids No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-461255
American Medical Association (AMA)
Fukunaga, Keisuke& Taki, Masumi. Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-461255
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461255