Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient ObOb Mice
Joint Authors
Ortinau, Laura C.
Ruebel, Meghan L.
Meers, Grace M.
Perfield, James W.
Rector, R. Scott
Pickering, R. Taylor
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nonalcoholic fatty liver disease (NAFLD) is strongly linked to obesity, insulin resistance, and abnormal hepatic lipid metabolism; however, the precise regulation of these processes remains poorly understood.
Here we examined genes and proteins involved in hepatic oxidation and lipogenesis in 14-week-old leptin-deficient Ob/Ob mice, a commonly studied model of obesity and hepatic steatosis.
Obese Ob/Ob mice had increased fasting glucose, insulin, and calculated HOMA-IR as compared with lean wild-type (WT) mice.
Ob/Ob mice also had greater liver weights, hepatic triglyceride (TG) content, and markers of de novo lipogenesis, including increased hepatic gene expression and protein content of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD-1), as well as elevated gene expression of PPARγ and SREBP-1c compared with WT mice.
While hepatic mRNA levels for PGC-1α, PPARα, and TFAM were elevated in Ob/Ob mice, measures of mitochondrial function (β-HAD activity and complete (to CO2) and total mitochondrial palmitate oxidation) and mitochondrial OXPHOS protein subunits I, III, and V content were significantly reduced compared with WT animals.
In summary, reduced hepatic mitochondrial content and function and an upregulation in de novo lipogenesis contribute to obesity-associated NAFLD in the leptin-deficient Ob/Ob mouse.
American Psychological Association (APA)
Perfield, James W.& Ortinau, Laura C.& Pickering, R. Taylor& Ruebel, Meghan L.& Meers, Grace M.& Rector, R. Scott. 2013. Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient ObOb Mice. Journal of Obesity،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-461338
Modern Language Association (MLA)
Perfield, James W.…[et al.]. Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient ObOb Mice. Journal of Obesity No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-461338
American Medical Association (AMA)
Perfield, James W.& Ortinau, Laura C.& Pickering, R. Taylor& Ruebel, Meghan L.& Meers, Grace M.& Rector, R. Scott. Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient ObOb Mice. Journal of Obesity. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-461338
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461338