Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity
Joint Authors
Girardi, C.
Celotti, L.
Casara, S.
Mognato, M.
Calura, Enrica
De Pittà, C.
Romualdi, C.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-23
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
We analyzed miRNA and mRNA expression profiles in human peripheral blood lymphocytes (PBLs) incubated in microgravity condition, simulated by a ground-based rotating wall vessel (RWV) bioreactor.
Our results show that 42 miRNAs were differentially expressed in MMG-incubated PBLs compared with 1 g incubated ones.
Among these, miR-9-5p, miR-9-3p, miR-155-5p, miR-150-3p, and miR-378-3p were the most dysregulated.
To improve the detection of functional miRNA-mRNA pairs, we performed gene expression profiles on the same samples assayed for miRNA profiling and we integrated miRNA and mRNA expression data.
The functional classification of miRNA-correlated genes evidenced significant enrichment in the biological processes of immune/inflammatory response, signal transduction, regulation of response to stress, regulation of programmed cell death, and regulation of cell proliferation.
We identified the correlation of miR-9-3p, miR-155-5p, miR-150-3p, and miR-378-3p expression with that of genes involved in immune/inflammatory response (e.g., IFNG and IL17F), apoptosis (e.g., PDCD4 and PTEN), and cell proliferation (e.g., NKX3-1 and GADD45A).
Experimental assays of cell viability and apoptosis induction validated the results obtained by bioinformatics analyses demonstrating that in human PBLs the exposure to reduced gravitational force increases the frequency of apoptosis and decreases cell proliferation.
American Psychological Association (APA)
Girardi, C.& De Pittà, C.& Casara, S.& Calura, Enrica& Romualdi, C.& Celotti, L.…[et al.]. 2014. Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity. BioMed Research International،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-461345
Modern Language Association (MLA)
Girardi, C.…[et al.]. Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity. BioMed Research International No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-461345
American Medical Association (AMA)
Girardi, C.& De Pittà, C.& Casara, S.& Calura, Enrica& Romualdi, C.& Celotti, L.…[et al.]. Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-461345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461345