Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles
Joint Authors
Sahin, Fikrettin
Yazıcı, M. Müge
Kahraman, Mehmet
Kocagöz, Sesin
Culha, Mustafa
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Abstract EN
Whole bacterial cell characterization is critically important for fast bacterial identification.
Surface-enhanced Raman scattering (SERS) is proven to be powerful technique to serve such a goal.
In this study, the characterization of whole bacterial cells in the microwells constructed from colloidal silver nanoparticles (AgNPs) with “convective-assembly” method is reported.
The proper size of the microwells for the model bacteria, Escherichia coli and Staphylococcus cohnii, is determined to be 1.2 μm from their electron microscopy images.
A minimum dilution factor of 20 is necessary for the bacterial samples collected from growth media to diminish the bacterial aggregation to place the bacterial cells into the microwells.
The constructed microwell structures are tested for their bacterial SERS performance and compared to the SERS spectra obtained from the samples prepared with a simple mixing of bacteria and AgNPs for the same bacteria.
The results indicate that microwell structures not only improve the spectral quality but also increase the reproducibility of the SERS spectra.
American Psychological Association (APA)
Culha, Mustafa& Yazıcı, M. Müge& Kahraman, Mehmet& Sahin, Fikrettin& Kocagöz, Sesin. 2012. Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles. Journal of Nanotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-461433
Modern Language Association (MLA)
Culha, Mustafa…[et al.]. Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles. Journal of Nanotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-461433
American Medical Association (AMA)
Culha, Mustafa& Yazıcı, M. Müge& Kahraman, Mehmet& Sahin, Fikrettin& Kocagöz, Sesin. Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles. Journal of Nanotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-461433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461433