Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia
Joint Authors
Shao, Zonghong
Liu, Hong
Li, Lijuan
Wang, Honglei
Yu, Hong
Wang, Yihao
Liu, Hui
Chen, Jin
Fu, Rong
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases.
Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia might be mediated by autoantibodies.
Autoantibodies were detected on the membrane of various bone marrow (BM) hemopoietic cells by bone marrow mononuclear-cell-Coombs test or flow cytometric analysis.
Thus, the hemocytopenia was termed “Immunorelated Pancytopenia” (IRP) to distinguish it from other pancytopenias.
Autoantigens in IRP were investigated by membrane protein extraction from BM hemopoietic cells and BM supernatant from IRP patients.
Autoantibody IgG was detected in the BM supernatant of 75% of patients (15/20), which was significantly higher than that in aplastic anemia, myelodysplastic syndrome, or autoimmune hemolytic anemia patients (0%) and normal healthy controls (0%) (P<0.01).
Autoantigens had approximate molecular weights of 25, 30, 47.5, 60, 65, 70, and 80 kDa, some of which were further identified by mass fingerprinting.
This study identified that a G-protein-coupled receptor 156 variant and chain P, a crystal structure of the cytoplasmic domain of human erythrocyte band-3 protein, were autoantigens in IRP.
Further studies are needed to confirm the antigenicity of these autoantigens.
American Psychological Association (APA)
Liu, Hui& Fu, Rong& Wang, Yihao& Liu, Hong& Li, Lijuan& Wang, Honglei…[et al.]. 2013. Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461446
Modern Language Association (MLA)
Liu, Hui…[et al.]. Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia. Clinical and Developmental Immunology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-461446
American Medical Association (AMA)
Liu, Hui& Fu, Rong& Wang, Yihao& Liu, Hong& Li, Lijuan& Wang, Honglei…[et al.]. Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461446