Reduction of Cellular Lipid Content by a Knockdown of Drosophila PDP1γ and Mammalian Hepatic Leukemia Factor
Joint Authors
Manev, Hari
Dzitoyeva, Svetlana
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In exploring the utility of double-stranded RNA (dsRNA) injections for silencing the PAR-domain protein 1 (Pdp1) gene in adult Drosophila, we noticed a dramatic loss of fat tissue lipids.
To verify that our RNAi approach produced the expected Pdp1 knockdown, the abdominal fat tissues sections were stained with PDP1 antibodies.
PDP1 protein immunostaining was absent in flies injected with dsRNA targeting a sequence common to all known Pdp1 isoforms.
Subsequent experiments revealed that lipid staining is reduced in flies injected with dsRNA against Pdp1γ (fat body specific) and not against Pdp1ε (predominantly involved in circadian mechanisms).
Drosophila PDP1γ protein shows a high homology to mammalian thyrotroph embryonic factor (TEF), albumin D site-binding protein (DBP), and hepatic leukemia factor (HLF) transcription factors.
In an in vitro model of drug- (olanzapine-) induced adiposity in mouse 3T3-L1 cells, the mRNA content of HLF but not TEF and DBP was increased by the drug treatment.
A knockdown of the HLF mRNA by transfecting the cultures with HLF dsRNA significantly reduced their lipid content.
Furthermore, the HLF RNAi prevented olanzapine from increasing the cell lipid content.
These results suggest that the PDP1/HLF system may play a role in physiological and drug-influenced lipid regulation.
American Psychological Association (APA)
Dzitoyeva, Svetlana& Manev, Hari. 2013. Reduction of Cellular Lipid Content by a Knockdown of Drosophila PDP1γ and Mammalian Hepatic Leukemia Factor. Journal of Lipids،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461462
Modern Language Association (MLA)
Dzitoyeva, Svetlana& Manev, Hari. Reduction of Cellular Lipid Content by a Knockdown of Drosophila PDP1γ and Mammalian Hepatic Leukemia Factor. Journal of Lipids No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-461462
American Medical Association (AMA)
Dzitoyeva, Svetlana& Manev, Hari. Reduction of Cellular Lipid Content by a Knockdown of Drosophila PDP1γ and Mammalian Hepatic Leukemia Factor. Journal of Lipids. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461462