Comparative Effects of Biodynes, Tocotrienol-Rich Fraction, and Tocopherol in Enhancing Collagen Synthesis and Inhibiting Collagen Degradation in Stress-Induced Premature Senescence Model of Human Diploid Fibroblasts
Joint Authors
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Ngah, Wan Zurinah Wan
Ismail, Zahariah
Khor, Shy Cian
Jam, Faidruz Azura
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Biodynes, tocotrienol-rich fraction (TRF), and tocopherol have shown antiaging properties.
However, the combined effects of these compounds on skin aging are yet to be investigated.
This study aimed to elucidate the skin aging effects of biodynes, TRF, and tocopherol on stress-induced premature senescence (SIPS) model of human diploid fibroblasts (HDFs) by determining the expression of collagen and MMPs at gene and protein levels.
Primary HDFs were treated with biodynes, TRF, and tocopherol prior to hydrogen peroxide (H2O2) exposure.
The expression of COL1A1, COL3A1, MMP1, MMP2, MMP3, and MMP9 genes was determined by qRT-PCR.
Type I and type III procollagen proteins were measured by Western blotting while the activities of MMPs were quantified by fluorometric Sensolyte MMP Kit.
Our results showed that biodynes, TRF, and tocopherol upregulated collagen genes and downregulated MMP genes (P<0.05).
Type I procollagen and type III procollagen protein levels were significantly increased in response to biodynes, TRF, and tocopherol treatment (P<0.05) with reduction in MMP-1, MMP-2, MMP-3, and MMP-9 activities (P<0.05).
These findings indicated that biodynes, TRF, and tocopherol effectively enhanced collagen synthesis and inhibited collagen degradation and therefore may protect the skin from aging.
American Psychological Association (APA)
Makpol, Suzana& Jam, Faidruz Azura& Khor, Shy Cian& Ismail, Zahariah& Mohd Yusof, Yasmin Anum& Ngah, Wan Zurinah Wan. 2013. Comparative Effects of Biodynes, Tocotrienol-Rich Fraction, and Tocopherol in Enhancing Collagen Synthesis and Inhibiting Collagen Degradation in Stress-Induced Premature Senescence Model of Human Diploid Fibroblasts. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-461525
Modern Language Association (MLA)
Makpol, Suzana…[et al.]. Comparative Effects of Biodynes, Tocotrienol-Rich Fraction, and Tocopherol in Enhancing Collagen Synthesis and Inhibiting Collagen Degradation in Stress-Induced Premature Senescence Model of Human Diploid Fibroblasts. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-461525
American Medical Association (AMA)
Makpol, Suzana& Jam, Faidruz Azura& Khor, Shy Cian& Ismail, Zahariah& Mohd Yusof, Yasmin Anum& Ngah, Wan Zurinah Wan. Comparative Effects of Biodynes, Tocotrienol-Rich Fraction, and Tocopherol in Enhancing Collagen Synthesis and Inhibiting Collagen Degradation in Stress-Induced Premature Senescence Model of Human Diploid Fibroblasts. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-461525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461525