Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy
Joint Authors
Rognoni, Paola
Comincini, Sergio
Azzalin, Alberto
Miracco, Clelia
Chiarelli, Laurent R.
Valentini, Giovanna
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Doppel (Dpl) is a membrane-bound glycoprotein mainly expressed in the testis of adult healthy people.
It is generally absent in the central nervous system, but its coding gene sequence is ectopically expressed in astrocytoma specimens and in derived cell lines.
In this paper, we investigated the expression and the biochemical features of Dpl in a panel of 49 astrocytoma specimens of different WHO malignancy grades.
As a result, Dpl was expressed in the majority of the investigated specimens (86%), also including low grade samples.
Importantly, Dpl exhibited different cellular localizations and altered glycan moieties composition, depending on the tumor grade.
Most low-grade astrocytomas (83%) showed a membrane-bound Dpl, like human healthy testis tissue, whereas the majority of high-grade astrocytomas (75%) displayed a cytosolic Dpl.
Deglycosylation studies with N-glycosidase F and/or neuraminidase highlighted defective glycan moieties and an unexpected loss of sialic acid.
To find associations between glial tumor progression and Dpl biochemical features, predictive bioinformatics approaches were produced.
In particular, Decision tree and Nomogram analysis showed well-defined Dpl-based criteria that separately clustered low-and high-grade astrocytomas.
Taken together, these findings show that in astrocytomas, Dpl undergoes different molecular processes that might constitute additional helpful tools to characterize the glial tumor progression.
American Psychological Association (APA)
Rognoni, Paola& Chiarelli, Laurent R.& Comincini, Sergio& Azzalin, Alberto& Miracco, Clelia& Valentini, Giovanna. 2010. Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-461568
Modern Language Association (MLA)
Rognoni, Paola…[et al.]. Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-461568
American Medical Association (AMA)
Rognoni, Paola& Chiarelli, Laurent R.& Comincini, Sergio& Azzalin, Alberto& Miracco, Clelia& Valentini, Giovanna. Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-461568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461568