Advanced Corneal Cross-Linking System with Fluorescence Dosimetry

Abstract EN

Purpose.

This paper describes an advanced system that combines corneal cross-linking with riboflavin with fluorescence dosimetry, the ability to measure riboflavin diffusion within the cornea both before and during UVA treatment.

Methods and Results.

A corneal cross-linking system utilizing a digital micromirror device (DMD) was assembled and used to measure diffusion coefficients of 0.1% riboflavin in 20% dextran in porcine eyes.

A value of (3.3±0.2)×10−7 cm2/s was obtained for the stroma.

Diffusion coefficients for the transepithelial formulation of 0.1% riboflavin in 0.44% saline and 0.02% BAK were also measured to be 4.7±0.3×10−8 cm2/s for epithelium only and (4.6±0.4)×10−7 cm2/s for stroma only.

Riboflavin consumption during a UVA treatment was also demonstrated.

Conclusion.

A new advanced corneal cross-linking system with fluorescence dosimetry of riboflavin has been demonstrated.

It is hoped that this method may play a significant role in determining the underlying mechanisms of corneal cross-linking and assist with the development of additional riboflavin formulations.

Moreover, dosimetry may prove valuable in providing a method to account for the biological differences between individuals, potentially informing cornea-specific UVA treatment doses in real time.