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Targeting the OB-Folds of Replication Protein A with Small Molecules
Joint Authors
Fitch, Richard W.
Earley, Jennifer N.
Turchi, John J.
Georgiadis, Millie M.
Anciano Granadillo, Victor J.
Shuck, Sarah C.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Replication protein A (RPA) is the main eukaryotic single-strand (ss) DNA-binding protein involved in DNA replication and repair.
We have identified and developed two classes of small molecule inhibitors (SMIs) that show in vitro inhibition of the RPA-DNA interaction.
We present further characterization of these SMIs with respect to their target binding, mechanism of action, and specificity.
Both reversible and irreversible modes of inhibition are observed for the different classes of SMIs with one class found to specifically interact with DNA-binding domains A and B (DBD-A/B) of RPA.
In comparison with other oligonucleotide/oligosaccharide binding-fold (OB-fold) containing ssDNA-binding proteins, one class of SMIs displayed specificity for the RPA protein.
Together these data demonstrate that the specific targeting of a protein-DNA interaction can be exploited towards interrogating the cellular activity of RPA as well as increasing the efficacy of DNA-damaging chemotherapeutics used in cancer treatment.
American Psychological Association (APA)
Anciano Granadillo, Victor J.& Earley, Jennifer N.& Shuck, Sarah C.& Georgiadis, Millie M.& Fitch, Richard W.& Turchi, John J.. 2010. Targeting the OB-Folds of Replication Protein A with Small Molecules. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-461834
Modern Language Association (MLA)
Anciano Granadillo, Victor J.…[et al.]. Targeting the OB-Folds of Replication Protein A with Small Molecules. Journal of Nucleic Acids No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-461834
American Medical Association (AMA)
Anciano Granadillo, Victor J.& Earley, Jennifer N.& Shuck, Sarah C.& Georgiadis, Millie M.& Fitch, Richard W.& Turchi, John J.. Targeting the OB-Folds of Replication Protein A with Small Molecules. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-461834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461834