Determination of Tetracycline in Pharmaceutical Preparation by Molecular and Atomic Absorption Spectrophotometry and High Performance Liquid Chromatography via Complex Formation with Au(III) and Hg(II) Ions in Solutions
Joint Authors
Hassan, Abbas Shebeeb
Abdulghani, Ahlam Jameel
Jasim, Hadi Hassan
Source
International Journal of Analytical Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
UV-visible and atomic spectrophotometry and HPLC techniques were applied for the determination of tetracycline (TC) in pharmaceutical preparations via complexation of the drug with Au(III) and Hg(II) ions in solutions.
The mole ratio of TC to metal ions was 1 : 1.
Maximum peak absorption at λ 425 and 320 nm for the two ions, respectively, was optimized at heating temperature 75°C for 15 minutes at pH = 4 followed by the extraction with ethyl acetate.
The percentage of extraction and stability constants for the two complexes was 95.247, 95.335% and 2.518×104, 1.162×105 M−1, respectively.
HPLC method was applied without extraction process.
The analytical data obtained from direct calibration curves of UV-visible absorption, FAAS, and HPLC for Au(III) complexes were recovery (100.78, 104.85, and 101.777%, resp.); detection limits (0.7403, 0.0997, and 2.647 μg/ml, resp.); linearity (5–70, 5–30, and 10–150 μg/ml, resp.), and correlation coefficient (0.9991, 0.9967, and 0.9986, resp.).
The analytical data obtained from direct calibration curves for Hg(II) complexes by UV-visible spectrophotometry and HPLC were recovery (100.95 and 102.000%, resp.); detection limits (0.5867 and 2.532 μg/ml, resp.); linearity (5–70 and 10–150 μg/ml, resp.); and correlation coefficients (0.9989 and 0.9997, resp.).
American Psychological Association (APA)
Abdulghani, Ahlam Jameel& Jasim, Hadi Hassan& Hassan, Abbas Shebeeb. 2013. Determination of Tetracycline in Pharmaceutical Preparation by Molecular and Atomic Absorption Spectrophotometry and High Performance Liquid Chromatography via Complex Formation with Au(III) and Hg(II) Ions in Solutions. International Journal of Analytical Chemistry،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-461920
Modern Language Association (MLA)
Abdulghani, Ahlam Jameel…[et al.]. Determination of Tetracycline in Pharmaceutical Preparation by Molecular and Atomic Absorption Spectrophotometry and High Performance Liquid Chromatography via Complex Formation with Au(III) and Hg(II) Ions in Solutions. International Journal of Analytical Chemistry No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-461920
American Medical Association (AMA)
Abdulghani, Ahlam Jameel& Jasim, Hadi Hassan& Hassan, Abbas Shebeeb. Determination of Tetracycline in Pharmaceutical Preparation by Molecular and Atomic Absorption Spectrophotometry and High Performance Liquid Chromatography via Complex Formation with Au(III) and Hg(II) Ions in Solutions. International Journal of Analytical Chemistry. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-461920
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461920