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Mitochondrial PCR-RFLP Assay to Distinguish Triatoma brasiliensis macromelasoma from Triatoma brasiliensis brasiliensis Subspecies (Hemiptera : Reduviidae)
Joint Authors
Obara, Marcos Takashi
Ceretti-Junior, Walter
Marrelli, Mauro Toledo
Vendrami, Daniel Pagotto
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Triatoma brasiliensis sensu lato (s.l.), the main vector of Chagas disease in northeastern Brazil, is a species complex comprising four species, one with two subspecies (T.
brasiliensis brasiliensis, T.
brasiliensis macromelasoma, T.
juazeirensis, T.
sherlocki, and T.
melanica), and each taxon displaying distinct ecological requirements.
In order to evaluate the genetic relationships among nine T.
brasiliensis s.l.
populations from northeastern Brazil, we analyzed their mitochondrial cytochrome c oxidase subunit 1 sequences and suggested a PCR-RFLP assay to distinguish between T.
b.
macromelasoma and T.
b.
brasiliensis subspecies.
All the specimens were morphologically identified as T.
b.
brasiliensis.
The resulting phylogenies identified two major clades that are congruent with the geographical populations studied.
Based on collection sites and in accordance with type-location, one clade was identified as the subspecies T.
b.
macromelasoma.
The second clade grouped T.
b.
brasiliensis populations.
Restriction endonuclease sites were observed in the sequences and used in PCR-RFLP assays, producing distinct fingerprints for T.
b.
macromelasoma and T.
b.
brasiliensis populations.
The results suggest that these are different species and that gene flow occurs only among T.
b.
brasiliensis populations, possibly associated with human activity in the area.
American Psychological Association (APA)
Vendrami, Daniel Pagotto& Ceretti-Junior, Walter& Obara, Marcos Takashi& Marrelli, Mauro Toledo. 2013. Mitochondrial PCR-RFLP Assay to Distinguish Triatoma brasiliensis macromelasoma from Triatoma brasiliensis brasiliensis Subspecies (Hemiptera : Reduviidae). Journal of Tropical Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461929
Modern Language Association (MLA)
Vendrami, Daniel Pagotto…[et al.]. Mitochondrial PCR-RFLP Assay to Distinguish Triatoma brasiliensis macromelasoma from Triatoma brasiliensis brasiliensis Subspecies (Hemiptera : Reduviidae). Journal of Tropical Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-461929
American Medical Association (AMA)
Vendrami, Daniel Pagotto& Ceretti-Junior, Walter& Obara, Marcos Takashi& Marrelli, Mauro Toledo. Mitochondrial PCR-RFLP Assay to Distinguish Triatoma brasiliensis macromelasoma from Triatoma brasiliensis brasiliensis Subspecies (Hemiptera : Reduviidae). Journal of Tropical Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461929
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461929