The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces : Gene Expression and Morphological Evaluation
Joint Authors
Jimbo, Ryo
Marcantonio, Rosemary Adriana Chiérici
Faeda, Rafael Silveira
Wennerberg, Ann
Coelho, Paulo Guilherme
Kobayashi, Fumio
Hayashi, Mariko
Mustafa, Kamal
Xue, Ying
Ono, Daisuke
Bougas, Kostas
Schwartz-Filho, Humberto Osvaldo
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim.
This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model.
Materials and Methods.
Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae.
Noncoated implants were used as controls.
After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR).
Results.
SEM revealed bony tissue attachment for both control and test implants.
Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control.
All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold).
The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls.
Conclusion.
The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.
American Psychological Association (APA)
Schwartz-Filho, Humberto Osvaldo& Bougas, Kostas& Coelho, Paulo Guilherme& Xue, Ying& Hayashi, Mariko& Faeda, Rafael Silveira…[et al.]. 2012. The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces : Gene Expression and Morphological Evaluation. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-461971
Modern Language Association (MLA)
Schwartz-Filho, Humberto Osvaldo…[et al.]. The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces : Gene Expression and Morphological Evaluation. International Journal of Biomaterials No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-461971
American Medical Association (AMA)
Schwartz-Filho, Humberto Osvaldo& Bougas, Kostas& Coelho, Paulo Guilherme& Xue, Ying& Hayashi, Mariko& Faeda, Rafael Silveira…[et al.]. The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces : Gene Expression and Morphological Evaluation. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-461971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461971