A New Candidate Substrate for Cell-Matrix Adhesion Study : The Acellular Human Amniotic Matrix
Joint Authors
Guo, Qianchen
Xue, Yuan
Zhao, Huajian
Lu, Xuya
Zhao, Xiaotao
Zheng, Hong
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In vivo adhesions between cells and the extracellular matrix play a crucial role in cell differentiation, proliferation, and migration as well as tissue remodeling.
Natural three-dimensional (3D) matrices, such as self-assembling matrices and Matrigel, have limitations in terms of their biomechanical properties.
Here, we present a simple method to produce an acellular human amniotic matrix (AHAM) with preserved biomechanical properties and a favorable adhesion potential.
On the stromal side of the AHAM, human foreskin fibroblasts (HFFs) attached and extended with bipolar spindle-shaped morphology proliferated to multilayer networks, invaded into the AHAM, and migrated in a straight line.
Moreover, αV integrin, paxillin, and fibronectin were observed to colocalize after 24 h of HFF culture on the stromal side of the AHAM.
Our results indicate that the AHAM may be an ideal candidate as a cell-matrix adhesion substrate to study cell adhesion and invasion as well as other functions in vitro under a tensile force that mimics the in vivo environment.
American Psychological Association (APA)
Guo, Qianchen& Lu, Xuya& Xue, Yuan& Zheng, Hong& Zhao, Xiaotao& Zhao, Huajian. 2012. A New Candidate Substrate for Cell-Matrix Adhesion Study : The Acellular Human Amniotic Matrix. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-462019
Modern Language Association (MLA)
Guo, Qianchen…[et al.]. A New Candidate Substrate for Cell-Matrix Adhesion Study : The Acellular Human Amniotic Matrix. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-462019
American Medical Association (AMA)
Guo, Qianchen& Lu, Xuya& Xue, Yuan& Zheng, Hong& Zhao, Xiaotao& Zhao, Huajian. A New Candidate Substrate for Cell-Matrix Adhesion Study : The Acellular Human Amniotic Matrix. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-462019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462019