Selectivity of Inhibition of N-Succinyl-l,l-Diaminopimelic Acid Desuccinylase in Bacteria : The product of dapE-gene Is Not the Target of l-Captopril Antimicrobial Activity
Joint Authors
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Topics
- Chemistry
- Biology
- Biotechnology
- Biochemistry
- Drugs
- Antibiotics
- Proteins
- Enzymes(Biology)
- Angiotensin-converting enzyme
- Alpha blocker
Abstract EN
The emergence of bacterial strains that are resistant to virtually all currently available antibiotics underscores the importance of developing new antimicrobial compounds.
N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE) is a metallohydrolase involved in the meso-diaminopimelate (mDAP)/lysine biosynthetic pathway necessary for lysine biosynthesis and for building the peptidoglycan cell wall.
Because DapE is essential for Gram-negative and some Gram-positive bacteria, DapE has been proposed as a good target for antibiotic development.
Recently, l-captopril has been suggested as a lead compound for inhibition of DapE, although its selectivity for this enzyme target in bacteria remains unclear (Gillner et al.
(2009)).
Here, we tested the selectivity of l-captopril against DapE in bacteria.
Since DapE knockout strains of gram-negative bacteria are viable upon chemical supplementation with mDAP, we reasoned that the antimicrobial activity of compounds targeting DapE should be abolished in mDAP-containing media.
Although l-captopril had modest antimicrobial activity in Escherichia coli and in Salmonella enterica, to our surprise, inhibition of bacterial growth was independent both of mDAP supplementation and DapE over-expression.
We conclude that DapE is not the main target of l-captopril inhibition in these bacteria.
The methods implemented here will be useful for screening DapE-selective antimicrobial compounds directly in bacterial cultures.
American Psychological Association (APA)
Uda, Narasimha Rao& Creus, Marc. 2011. Selectivity of Inhibition of N-Succinyl-l,l-Diaminopimelic Acid Desuccinylase in Bacteria : The product of dapE-gene Is Not the Target of l-Captopril Antimicrobial Activity. Bioinorganic Chemistry and Applications،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-462055
Modern Language Association (MLA)
Uda, Narasimha Rao& Creus, Marc. Selectivity of Inhibition of N-Succinyl-l,l-Diaminopimelic Acid Desuccinylase in Bacteria : The product of dapE-gene Is Not the Target of l-Captopril Antimicrobial Activity. Bioinorganic Chemistry and Applications No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-462055
American Medical Association (AMA)
Uda, Narasimha Rao& Creus, Marc. Selectivity of Inhibition of N-Succinyl-l,l-Diaminopimelic Acid Desuccinylase in Bacteria : The product of dapE-gene Is Not the Target of l-Captopril Antimicrobial Activity. Bioinorganic Chemistry and Applications. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-462055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462055