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Kinome Profiling
Author
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Diseases
Abstract EN
The use of arrays in genomics has led to a fast and reliable way to screen the transcriptome of an organism.
It can be automated and analysis tools have become available and hence the technique has become widely used within the past few years.
Signal-transduction routes rely mainly on the phosphorylation status of already available proteins; therefore kinases are central players in signal-transduction routes.
The array technology can now also be used for the analysis of the kinome.
To enable array analysis, consensus peptides for kinases are spot on a solid support.
After incubation with cell lysates and in the presence of radioactive ATP, radioactive peptides can be visualized and the kinases that are active in the cells can be determined.
The present paper reviews comprehensively the different kinome array platforms available and results obtained hitherto using such platforms.
It will appear that this technology does not disappoint its high expectations and is especially powerful because of its species independence.
Nevertheless, improvements are still possible and I shall also sketch future possible directions.
American Psychological Association (APA)
Peppelenbosch, Maikel P.. 2012. Kinome Profiling. Scientifica،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-462085
Modern Language Association (MLA)
Peppelenbosch, Maikel P.. Kinome Profiling. Scientifica No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-462085
American Medical Association (AMA)
Peppelenbosch, Maikel P.. Kinome Profiling. Scientifica. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-462085
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462085