Towards Al3+-Induced Manganese-Containing Superoxide Dismutase Inactivation and Conformational Changes : An Integrating Study with Docking Simulations
Joint Authors
Yang, Jiang-Liu
Lü, Zhi-Rong
Qian, Guo-Ying
Shao, Xiangrong
Yin, Shang-Jun
Park, Daeui
Zhou, Hai-Meng
Young Chung, Hae
Si, Yue-Xiu
Zhang, Zi-Ping
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Superoxide dismutase (SOD, EC 1.15.1.1) plays an important antioxidant defense role in skins exposed to oxygen.
We studied the inhibitory effects of Al3+ on the activity and conformation of manganese-containing SOD (Mn-SOD).
Mn-SOD was significantly inactivated by Al3+ in a dose-dependent manner.
The kinetic studies showed that Al3+ inactivated Mn-SOD follows the first-order reaction.
Al3+ increased the degree of secondary structure of Mn-SOD and also disrupted the tertiary structure of Mn-SOD, which directly resulted in enzyme inactivation.
We further simulated the docking between Mn-SOD and Al3+ (binding energy for Dock 6.3: −14.07 kcal/mol) and suggested that ASP152 and GLU157 residues were predicted to interact with Al3+, which are not located in the Mn-contained active site.
Our results provide insight into the inactivation of Mn-SOD during unfolding in the presence of Al3+ and allow us to describe a ligand binding via inhibition kinetics combined with the computational prediction.
American Psychological Association (APA)
Yang, Jiang-Liu& Yin, Shang-Jun& Si, Yue-Xiu& Lü, Zhi-Rong& Shao, Xiangrong& Park, Daeui…[et al.]. 2011. Towards Al3+-Induced Manganese-Containing Superoxide Dismutase Inactivation and Conformational Changes : An Integrating Study with Docking Simulations. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-462156
Modern Language Association (MLA)
Yang, Jiang-Liu…[et al.]. Towards Al3+-Induced Manganese-Containing Superoxide Dismutase Inactivation and Conformational Changes : An Integrating Study with Docking Simulations. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-462156
American Medical Association (AMA)
Yang, Jiang-Liu& Yin, Shang-Jun& Si, Yue-Xiu& Lü, Zhi-Rong& Shao, Xiangrong& Park, Daeui…[et al.]. Towards Al3+-Induced Manganese-Containing Superoxide Dismutase Inactivation and Conformational Changes : An Integrating Study with Docking Simulations. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-462156
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462156