Influence of Block of NF-Kappa B Signaling Pathway on Oxidative Stress in the Liver Homogenates

Abstract EN

The aim of the present study was to assess whether BAY 11-7082, a nuclear factor-kappaB (NF-κB) inhibitor, influences the level of reactive oxygen species (ROS), tumor necrosis factor alpha (TNF-α), and NF-κB related signaling pathways in the liver.

The animals were divided into 4 groups: I: saline; II: saline + endothelin-1 (ET-1) (1.25 μg/kg b.w., i.v.); III: saline + ET-1 (12.5 μg/kg b.w., i.v.); and IV: BAY 11-7082 (10 mg/kg b.w., i.v.) + ET-1 (12.5 μg/kg b.w., i.v.).

Injection of ET-1 alone at a dose of 12.5 μg/kg b.w.

showed a significant (P<0.001) increase in thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) level and decrease (P<0.01) in GSH level (vs.

control).

ET-1 administration slightly downregulated gene expression of p65 of NF-κB but potently and in a dose-dependent way downregulated p21-cip gene expression in the liver.

BAY 11-7082 significantly decreased TBARS (P<0.001), H2O2 (P<0.01) and improved the redox status (P<0.05), compared to ET-1 group.

The concentration of TNF-α was increased in the presence of ET-1 (P<0.05), while BAY 11-7082 decreased TNF-α concentration (P<0.01).

Inhibition of IkBα before ET-1 administration downregulated gene expression of p21-cip but had no effect on p65.