IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes
Joint Authors
Tanaka, Hideki
Suzuki, Naoto
Honda, Kazuhiro
Maeno, Masao
Namba, Aki
Watanabe, Yusuke
Aida, Yukiko
Matsumura, Hideo
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Elevated interleukin (IL)-1 concentrations in synovial fluid have been implicated in joint bone and cartilage destruction.
Previously, we showed that IL-1β stimulated the expression of prostaglandin (PG) receptor EP4 via increased PGE2 production.
However, the effect of IL-1β on osteoclast formation via chondrocytes is unclear.
Therefore, we examined the effect of IL-1β and/or celecoxib on the expression of macrophage colony-stimulating factor (M-CSF), receptor activator of NF-κB ligand (RANKL), and osteoprotegerin (OPG) in human chondrocytes, and the indirect effect of IL-1β on osteoclast-like cell formation using RAW264.7 cells.
OPG and RANKL expression increased with IL-1β; whereas M-CSF expression decreased.
Celecoxib blocked the stimulatory effect of IL-1β.
Conditioned medium from IL-1β-treated chondrocytes decreased TRAP staining in RAW264.7 cells.
These results suggest that IL-1β suppresses the formation of osteoclast-like cells via increased OPG production and decreased M-CSF production in chondrocytes, and OPG production may increase through an autocrine mechanism involving celecoxib-related PGs.
American Psychological Association (APA)
Watanabe, Yusuke& Namba, Aki& Aida, Yukiko& Honda, Kazuhiro& Tanaka, Hideki& Suzuki, Naoto…[et al.]. 2010. IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-462285
Modern Language Association (MLA)
Watanabe, Yusuke…[et al.]. IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes. Mediators of Inflammation No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-462285
American Medical Association (AMA)
Watanabe, Yusuke& Namba, Aki& Aida, Yukiko& Honda, Kazuhiro& Tanaka, Hideki& Suzuki, Naoto…[et al.]. IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes. Mediators of Inflammation. 2010. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-462285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462285