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Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef
Joint Authors
Gugsa, Likyelesh
Kumlehn, Jochen
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Information Technology and Computer Science
Abstract EN
Tef (Eragrostis tef) provides a major source of human nutrition in the Horn of Africa, but biotechnology has had little impact on its improvement to date.
Here, we report the elaboration of an in vitro regeneration protocol, based on the use of immature zygotic embryos as explant.
Explant size was an important determinant of in vitro regeneration efficiency, as was the formulation of the culture medium.
Optimal results were obtained by culturing 0.2–0.35 mm embryo explants on a medium containing KBP minerals, 9.2–13.8 μM 2,4-dichlorophenoxyacetic acid, 6 mM glutamine, and 0.5% Phytagel.
Although this protocol was effective for both the improved cultivar “DZ-01-196” and the landrace “Fesho”, the former produced consistently more embryogenic tissue and a higher number of regenerants.
An average of more than 2,800 shoots could be obtained from each “DZ-01-196” explant after 12 weeks of in vitro culture.
These shoots readily formed roots, and plantlets transferred to soil were able to develop into morphologically normal, fertile plants.
This regeneration and multiplication system should allow for the application of a range of biotechnological methods to tef.
American Psychological Association (APA)
Gugsa, Likyelesh& Kumlehn, Jochen. 2011. Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-462405
Modern Language Association (MLA)
Gugsa, Likyelesh& Kumlehn, Jochen. Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef. Biotechnology Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-462405
American Medical Association (AMA)
Gugsa, Likyelesh& Kumlehn, Jochen. Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-462405
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462405