Separation of Cyclic Dipeptides (Diketopiperazines) from Their Corresponding Linear Dipeptides by RP-HPLC and Method Validation
Joint Authors
Perzborn, Mareike
Syldatk, Christoph
Rudat, Jens
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Simple, rapid, sensitive, precise, and accurate methods for detection and separation of seven diketopiperazines (DKPs), cyclo(Gly-Gly), cyclo(dl-Ala-dl-Ala), cyclo(l-Asp-l-Phe), cyclo(l-Asp-l-Asp), cyclo(Gly-l-Phe), cyclo(l-Pro-l-Tyr), and cyclo(l-Arg-l-Arg), from their corresponding linear dipeptides and related amino acids l-Phe and l-Tyr by reversed-phase high-performance liquid chromatography (RP-HPLC) were established.
Moreover, for the racemic DKP cyclo(dl-Ala-dl-Ala) and dipeptide dl-Ala-dl-Ala, separation of the diastereomers was achieved.
All methods can be performed within 15 min.
For all DKPs, dipeptides, and amino acids, linear ranges with correlation coefficients R2 greater than 0.998 were determined.
Lowest limits of detection were found to be between 0.05 and 10 nmol per 10 μL injection, depending on the substance.
For all tested substances intrarun and interrun precision ranged from 0.5 to 4.7% and 0.7 to 9.9% relative standard deviation, and accuracy was between −4.2 and 8.1% relative error.
Short-term and freeze-thaw stabilities were 93% or greater for all substances.
Recovery rate after heat treatment was determined to be at least 97%.
These methods will be useful for quantitative determination of DKPs and their potential biodegradation products: dipeptides and amino acids
American Psychological Association (APA)
Perzborn, Mareike& Syldatk, Christoph& Rudat, Jens. 2013. Separation of Cyclic Dipeptides (Diketopiperazines) from Their Corresponding Linear Dipeptides by RP-HPLC and Method Validation. Chromatography Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-462433
Modern Language Association (MLA)
Perzborn, Mareike…[et al.]. Separation of Cyclic Dipeptides (Diketopiperazines) from Their Corresponding Linear Dipeptides by RP-HPLC and Method Validation. Chromatography Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-462433
American Medical Association (AMA)
Perzborn, Mareike& Syldatk, Christoph& Rudat, Jens. Separation of Cyclic Dipeptides (Diketopiperazines) from Their Corresponding Linear Dipeptides by RP-HPLC and Method Validation. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-462433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462433