Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus
Joint Authors
Vaish, Manisha
Singh, Vineet K.
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Nitric oxide and its derivative peroxynitrites are generated by host defense system to control bacterial infection.
However certain Gram positive bacteria including Staphylococcus aureus possess a gene encoding nitric oxide synthase (SaNOS) in their chromosome.
In this study it was determined that under normal growth conditions, expression of SaNOS was highest during early exponential phase of the bacterial growth.
In oxidative stress studies, deletion of SaNOS led to increased susceptibility of the mutant cells compared to wild-type S.
aureus.
While inhibition of SaNOS activity by the addition of L-NAME increased sensitivity of the wild-type S.
aureus to oxidative stress, the addition of a nitric oxide donor, sodium nitroprusside, restored oxidative stress tolerance of the SaNOS mutant.
The SaNOS mutant also showed reduced survival after phagocytosis by PMN cells with respect to wild-type S.
aureus.
American Psychological Association (APA)
Vaish, Manisha& Singh, Vineet K.. 2013. Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-462531
Modern Language Association (MLA)
Vaish, Manisha& Singh, Vineet K.. Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus. International Journal of Microbiology No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-462531
American Medical Association (AMA)
Vaish, Manisha& Singh, Vineet K.. Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-462531
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462531