Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells
Joint Authors
Sherley, James L.
Paré, Jean-François
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Previous efforts to improve the efficiency of cellular reprogramming for the generation of induced pluripotent stem cells (iPSCs) have focused mainly on transcription factors and small molecule combinations.
Here, we report the results of our focus instead on the phenotype of the cells targeted for reprogramming.
We find that adult mouse pancreatic tissue stem cells derived by the method of suppression of asymmetric cell kinetics (SACK) acquire increased potency simply by culture under conditions for the production and maintenance of pluripotent stem cells.
Moreover, supplementation with the SACK agent xanthine, which promotes symmetric self-renewal, significantly increases the efficiency and degree of acquisition of pluripotency properties.
In transplantation analyses, clonal reprogrammed pancreatic stem cells produce slow-growing tumors with tissue derivative of all three embryonic germ layers.
This acquisition of pluripotency, without transduction with exogenous transcription factors, supports the concept that tissue stem cells are predisposed to cellular reprogramming, particularly when symmetrically self-renewing.
American Psychological Association (APA)
Paré, Jean-François& Sherley, James L.. 2011. Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-462551
Modern Language Association (MLA)
Paré, Jean-François& Sherley, James L.. Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-462551
American Medical Association (AMA)
Paré, Jean-François& Sherley, James L.. Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-462551
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462551