Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway
Joint Authors
Kuo, Meng-Fai
Wang, Sheng-De
Yang, Shih-Hung
Kung, Hsiu-Ni
Syu, Jhih-Pu
Peng, Yu-Sen
Wang, Seu-Mei
Chen, Ying
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-05
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
Glioma is the most malignant tumor of the central nervous system.
Efforts on the development of new chemotherapy are mandatory.
Andrographolide (AND), a diterpenoid lactone isolated from the Andrographis paniculata, has been shown to have antitumor activities in several types of cancer cells.
Whether AND can exert its antitumor activity in glioblastoma cells remains unknown.
This study examined the anticancer effects of AND, both in vitro and in vivo.
Methods.
Cell apoptosis was assayed by flow cytometry and nuclear staining.
The signaling pathway for AND was determined by western blotting.
The effects of AND on tumor growth was evaluated in a mouse model.
Results and Conclusion.
In vitro, with application of specific inhibitors and siRNA, AND-induced apoptosis was proven through ROS-ERK-P53-caspase 7-PARP signaling pathway.
In vivo, AND significantly retarded tumor growth and caused regression of well-formed tumors in vivo.
Furthermore, AND did not induce apoptosis or activate ERK and p53 in primary cultured astrocyte cells, and it may serve as a potential therapeutic candidate for the treatment of glioma.
American Psychological Association (APA)
Yang, Shih-Hung& Wang, Seu-Mei& Syu, Jhih-Pu& Chen, Ying& Wang, Sheng-De& Peng, Yu-Sen…[et al.]. 2014. Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway. BioMed Research International،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-462605
Modern Language Association (MLA)
Yang, Shih-Hung…[et al.]. Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway. BioMed Research International No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-462605
American Medical Association (AMA)
Yang, Shih-Hung& Wang, Seu-Mei& Syu, Jhih-Pu& Chen, Ying& Wang, Sheng-De& Peng, Yu-Sen…[et al.]. Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-462605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462605