The β-SiC Nanowires (~100 nm)‎ Induce Apoptosis via Oxidative Stress in Mouse Osteoblastic Cell Line MC3T3-E1

Abstract EN

Silicon carbide (SiC), a compound of silicon and carbon, with chemical formula SiC, the beta modification (β-SiC), with a zinc blende crystal structure (similar to diamond), is formed at temperature below 1700∘C.

β-SiC will be the most suitable ceramic material for the future hard tissue replacement, such as bone and tooth.

The in vitro cytotoxicity of β-SiC nanowires was investigated for the first time.

Our results indicated that 100 nm long SiC nanowires could significantly induce the apoptosis in MC3T3-E1 cells, compared with 100 μm long SiC nanowires.

And 100 nm long SiC nanowires increased oxidative stress in MC3T3-E1 cells, as determined by the concentrations of MDA (as a marker of lipid peroxidation) and 8-OHdG (indicator of oxidative DNA damage).

Moreover, transmission electron microscopy (TEM) was performed to evaluate the morphological changes of MC3T3-E1 cells.

After treatment with 100 nm long SiC nanowires, the mitochondria were swelled and disintegrated, and the production of ATP and the total oxygen uptake were also decreased significantly.

Therefore, β-SiC nanowires may have limitations as medical material.