The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

Abstract EN

Introduction.

Acute kidney injury (AKI) pathogenesis is complex.

Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients.

Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis.

We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI.

Methods.

Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D3 0.4 mcg/kg/day subcutaneously for 8 days.

Blood pressures and 24-hour urine were measured.

Blood urea and creatinine levels and urine tubular injury markers were measured.

Renal histology was semiquantitatively assessed.

Results.

Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model.

Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group.

Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups.

Conclusions.

Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.