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Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop
Joint Authors
Boe, A.
Gonzalez-Hernandez, J. L.
Gedye, K. R.
Owens, V.
Source
International Journal of Plant Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Prairie cordgrass (Spartina pectinata Bosc ex Link) is an indigenous, perennial grass of North America that is being developed into a cellulosic biomass crop suitable for biofuel production.
Limited research has been performed into the breeding of prairie cordgrass; this research details an initial investigation into the development of a breeding program for this species.
Genomic libraries enriched for four simple sequence repeat (SSR) motifs were developed, 25 clones from each library were sequenced, identifying 70 SSR regions, and primers were developed for these regions, 35 of which were amplified under standard PCR conditions.
These SSR markers were used to validate the crossing methodology of prairie cordgrass and it was found that crosses between two plants occurred without the need for emasculation.
The successful cross between two clones of prairie cordgrass indicates that this species is not self-incompatible.
The results from this research will be used to instigate the production of a molecular map of prairie cordgrass which can be used to incorporate marker-assisted selection (MAS) protocols into a breeding program to improve this species for cellulosic biomass production.
American Psychological Association (APA)
Gedye, K. R.& Gonzalez-Hernandez, J. L.& Owens, V.& Boe, A.. 2012. Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop. International Journal of Plant Genomics،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-462647
Modern Language Association (MLA)
Gedye, K. R.…[et al.]. Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop. International Journal of Plant Genomics No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-462647
American Medical Association (AMA)
Gedye, K. R.& Gonzalez-Hernandez, J. L.& Owens, V.& Boe, A.. Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop. International Journal of Plant Genomics. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-462647
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462647