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Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques
Joint Authors
Tang, Qian
Cao, Hong-Yu
Peng, Xiao-Jun
Ma, Jing
Wang, Jing-Yun
Zheng, Xue-Fang
Yang, Yan-Jie
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
In order to investigate how the amino acids on the surface of myoglobin molecule influence myoglobin's structure and function, a variety of spectroscopy techniques were applied in the study of the interaction between Fe(III) and myoglobin (wild type and its mutants, D44K, D60K, and K56D).
The results demonstrate that Fe(III) can quench the fluorescence of wild type and mutants of myoglobin, and the quenching mechanisms are static quenching.
It is found that the binding distance between Fe(III) and myoglobin mutants gets smaller, the binding capacity increases by the values of binding constant and the bimolecular quenching constant as well as the binding distance.
Those data also indicate that the metal ion Fe(III) can interact strongly with myoglobin mutants.
The three-dimensional conformation change after surface amino acids are replaced is detected by the UV absorption spectroscopy and fluorescence spectroscopy, which make mutants become more dynamic and change its function and interaction with Fe(III) strongly.
American Psychological Association (APA)
Tang, Qian& Peng, Xiao-Jun& Cao, Hong-Yu& Yang, Yan-Jie& Ma, Jing& Wang, Jing-Yun…[et al.]. 2012. Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-462723
Modern Language Association (MLA)
Tang, Qian…[et al.]. Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques. Journal of Spectroscopy No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-462723
American Medical Association (AMA)
Tang, Qian& Peng, Xiao-Jun& Cao, Hong-Yu& Yang, Yan-Jie& Ma, Jing& Wang, Jing-Yun…[et al.]. Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques. Journal of Spectroscopy. 2012. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-462723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462723