Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus
Joint Authors
Zhang, Lixin
DeBusscher, Joan
Styka, Anne N.
Marrs, Carl F.
Li, Sheng
Pillai, Parvathy
Borchardt, Stephanie M.
Foxman, Betsy
Mehta, Puja
Reddi, Usha
Srinivasan, Usha
Source
Interdisciplinary Perspectives on Infectious Diseases
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-02-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Many bacterial species function as both commensals and pathogens; we used this dual nature to develop a high-throughput molecular epidemiological approach to identifying bacterial virulence genes.
We applied our approach to Group B Streptococcus (GBS).
Three representative commensal and one invasive GBS isolates were selected as tester strains from a population-based collection.
We used microarray-based comparative genomic hybridization to identify open reading frames (ORFs) present in two sequenced invasive strains, but absent or divergent in tester strains.
We screened 23 variable ORFs against 949 GBS isolates using a GBS Library on a Slide (LOS) microarray platform.
Four ORFs occurred more frequently in invasive than commensal isolates, and one appeared more frequently in commensal isolates.
Comparative hybridization using an oligonucleotide microarray, combined with epidemiologic screening using the LOS microarray platform, enabled rapid identification of bacterial genes potentially associated with pathogenicity.
American Psychological Association (APA)
Zhang, Lixin& Reddi, Usha& Srinivasan, Usha& Li, Sheng& Borchardt, Stephanie M.& Pillai, Parvathy…[et al.]. 2008. Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-462753
Modern Language Association (MLA)
Zhang, Lixin…[et al.]. Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus. Interdisciplinary Perspectives on Infectious Diseases No. 2008 (2008), pp.1-10.
https://search.emarefa.net/detail/BIM-462753
American Medical Association (AMA)
Zhang, Lixin& Reddi, Usha& Srinivasan, Usha& Li, Sheng& Borchardt, Stephanie M.& Pillai, Parvathy…[et al.]. Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus. Interdisciplinary Perspectives on Infectious Diseases. 2008. Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-462753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462753