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Identification of Residues Important for the Activity of Haloferax volcanii AglD, a Component of the Archaeal N-Glycosylation Pathway
Joint Authors
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In Haloferax volcanii, AglD adds the final hexose to the N-linked pentasaccharide decorating the S-layer glycoprotein.
Not knowing the natural substrate of the glycosyltransferase, together with the challenge of designing assays compatible with hypersalinity, has frustrated efforts at biochemical characterization of AglD activity.
To circumvent these obstacles, an in vivo assay designed to identify amino acid residues important for AglD activity is described.
In the assay, restoration of AglD function in an Hfx.
volcanii aglD deletion strain transformed to express plasmid-encoded versions of AglD, generated through site-directed mutagenesis at positions encoding residues conserved in archaeal homologues of AglD, is reflected in the behavior of a readily detectable reporter of N-glycosylation.
As such Asp110 and Asp112 were designated as elements of the DXD motif of AglD, a motif that interacts with metal cations associated with nucleotide-activated sugar donors, while Asp201 was predicted to be the catalytic base of the enzyme.
American Psychological Association (APA)
Kaminski, Lina& Eichler, Jerry. 2010. Identification of Residues Important for the Activity of Haloferax volcanii AglD, a Component of the Archaeal N-Glycosylation Pathway. Archaea،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-462789
Modern Language Association (MLA)
Kaminski, Lina& Eichler, Jerry. Identification of Residues Important for the Activity of Haloferax volcanii AglD, a Component of the Archaeal N-Glycosylation Pathway. Archaea No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-462789
American Medical Association (AMA)
Kaminski, Lina& Eichler, Jerry. Identification of Residues Important for the Activity of Haloferax volcanii AglD, a Component of the Archaeal N-Glycosylation Pathway. Archaea. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-462789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462789