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Recombinant Human Factor IX Produced from Transgenic Porcine Milk
Joint Authors
Tu, Ching-Fu
Lin, Yin-Shen
Lee, Meng-Hwan
Yen, Chon-Ho
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Production of biopharmaceuticals from transgenic animal milk is a cost-effective method for highly complex proteins that cannot be efficiently produced using conventional systems such as microorganisms or animal cells.
Yields of recombinant human factor IX (rhFIX) produced from transgenic porcine milk under the control of the bovine α-lactalbumin promoter reached 0.25 mg/mL.
The rhFIX protein was purified from transgenic porcine milk using a three-column purification scheme after a precipitation step to remove casein.
The purified protein had high specific activity and a low ratio of the active form (FIXa).
The purified rhFIX had 11.9 γ-carboxyglutamic acid (Gla) residues/mol protein, which approached full occupancy of the 12 potential sites in the Gla domain.
The rhFIX was shown to have a higher isoelectric point and lower sialic acid content than plasma-derived FIX (pdFIX).
The rhFIX had the same N-glycosylation sites and phosphorylation sites as pdFIX, but had a higher specific activity.
These results suggest that rhFIX produced from porcine milk is physiologically active and they support the use of transgenic animals as bioreactors for industrial scale production in milk.
American Psychological Association (APA)
Lee, Meng-Hwan& Lin, Yin-Shen& Tu, Ching-Fu& Yen, Chon-Ho. 2014. Recombinant Human Factor IX Produced from Transgenic Porcine Milk. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-462805
Modern Language Association (MLA)
Lee, Meng-Hwan…[et al.]. Recombinant Human Factor IX Produced from Transgenic Porcine Milk. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-462805
American Medical Association (AMA)
Lee, Meng-Hwan& Lin, Yin-Shen& Tu, Ching-Fu& Yen, Chon-Ho. Recombinant Human Factor IX Produced from Transgenic Porcine Milk. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-462805
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462805