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Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy
Joint Authors
Huleihil, Mahmoud
Erukhimovitch, V.
Huleihel, M.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Fourier transform infrared microspectroscopy (FTIR-M) can detect small molecular changes in cells and therefore was previously applied for the identification of different biological samples.
In the present study, FTIR spectroscopy was used for the identification and discrimination of Vero cells infected with herpes viruses or contaminated with bacteria or fungi in cell culture.
Vero cells in culture were infected herpes simplex virus type 1 (HSV-1) or contaminated with E.
coli bacteria or Candida albicans fungi and analyzed by FTIR microscopy at 24 h postinfection/contamination.
Specific different spectral changes were observed according to the infecting or contaminating agent.
For instance, both pure fungi and cell culture contaminated with this fungi showed specific peaks at 1030 cm−1 and at 1373 cm−1 regions, while pure E.
coli and cell culture contaminated with this bacteria showed a specific and unique peak at 1657 cm−1.
These results support the potential of developing FTIR microspectroscopy as a simple, reagent free method for identification and discrimination between different tissue infection or contamination with various pathogens.
American Psychological Association (APA)
Erukhimovitch, V.& Huleihil, Mahmoud& Huleihel, M.. 2013. Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-463004
Modern Language Association (MLA)
Erukhimovitch, V.…[et al.]. Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy. Journal of Spectroscopy No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-463004
American Medical Association (AMA)
Erukhimovitch, V.& Huleihil, Mahmoud& Huleihel, M.. Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy. Journal of Spectroscopy. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-463004
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463004