Evaluation of Hepatic Mitochondria and Hematological Parameters in Zidovudine-Treated B6C3F1 Mice
Joint Authors
Branham, William S.
Moland, Carrie L.
Mittelstaedt, Roberta A.
Lee, Taewon
Lewis, Sherry M.
Fuscoe, James C.
Desai, Varsha G.
Leakey, Julian E. A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The effects of 12-week exposure to zidovudine (AZT) at 400, 500, and 600 mg/kg/d were examined on expression of 542 mitochondria-related genes and mitochondrial DNA (mtDNA) copy number in the liver of male and female B6C3F1 mice to understand mitochondrial role in sex-related differences in development of lactic acidosis.
Plasma lactate levels and hematologic parameters were also examined.
Results indicated increased red blood cell (RBC) count in vehicle-treated controls, whereas a dose-related decline in the RBC count was noted in AZT-treated mice compared to the basal levels before treatments began.
These decreases were associated with significant dose-related increases in mean corpuscular volume and mean corpuscular hemoglobin levels.
This effect was greater in AZT-treated females compared to males.
In both sexes, 12-week AZT or vehicle exposure significantly reduced plasma lactate levels compared to the basal levels.
Results also showed modest, but significant, changes in the expression of genes associated with apoptosis and lipid metabolism at 600 mg/kg/d AZT.
Neither drug nor sex influenced hepatic mtDNA copy number.
Altogether, 12-week AZT exposure as high as 600 mg/kg/d did not impair hepatic mitochondria or induce lactic acidosis in B6C3F1 mice.
However, AZT-mediated hematologic toxicity appeared to be greater in females compared to males.
American Psychological Association (APA)
Desai, Varsha G.& Lee, Taewon& Moland, Carrie L.& Branham, William S.& Mittelstaedt, Roberta A.& Lewis, Sherry M.…[et al.]. 2012. Evaluation of Hepatic Mitochondria and Hematological Parameters in Zidovudine-Treated B6C3F1 Mice. AIDS Research and Treatment،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-463037
Modern Language Association (MLA)
Desai, Varsha G.…[et al.]. Evaluation of Hepatic Mitochondria and Hematological Parameters in Zidovudine-Treated B6C3F1 Mice. AIDS Research and Treatment No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-463037
American Medical Association (AMA)
Desai, Varsha G.& Lee, Taewon& Moland, Carrie L.& Branham, William S.& Mittelstaedt, Roberta A.& Lewis, Sherry M.…[et al.]. Evaluation of Hepatic Mitochondria and Hematological Parameters in Zidovudine-Treated B6C3F1 Mice. AIDS Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-463037
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463037