Comparison of Long Noncoding RNA and mRNA Expression Profiles in Mesenchymal Stem Cells Derived from Human Periodontal Ligament and Bone Marrow
Joint Authors
Wang, Liping
Du, Juan
Wang, Songlin
Ding, Gang
Fan, Zhipeng
Dong, Rui
Wang, Jinsong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) in different anatomic locations possess diverse biological activities.
Maintaining the pluripotent state and differentiation depend on the expression and regulation of thousands of genes, but it remains unclear which molecular mechanisms underlie MSC diversity.
Thus, potential MSC applications are restricted.
Long noncoding RNAs (lncRNAs) are implicated in the complex molecular circuitry of cellular processes.
We investigated differences in lncRNA and mRNA expression profiles between bone marrow stem cells (BMSCs) and periodontal ligament stem cells (PDLSCs) with lncRNA microarray assays and bioinformatics analysis.
In PDLSCs, numerous lncRNAs were significantly upregulated (n=457) or downregulated (n=513) compared to BMSCs.
Furthermore, 1,578 mRNAs were differentially expressed.
These genes implicated cellular pathways that may be associated with MSC characteristics, including apoptosis, MAPK, cell cycle, and Wnt signaling pathway.
Signal-net analysis indicated that phospholipase C beta 4, filamin B beta, calcium/calmodulin-dependent protein kinase II gamma, and the ionotropic glutamate receptor, AMPA 1, had the highest betweenness centrality among significant genes in the differential gene profile network.
A comparison between the coding-noncoding gene coexpression networks of PDLSCs and BMSCs identified chemokine (C-X-C motif) ligand 12 as a core regulatory factor in MSC biology.
These results provided insight into the mechanisms underlying MSC biology.
American Psychological Association (APA)
Dong, Rui& Du, Juan& Wang, Liping& Wang, Jinsong& Ding, Gang& Wang, Songlin…[et al.]. 2014. Comparison of Long Noncoding RNA and mRNA Expression Profiles in Mesenchymal Stem Cells Derived from Human Periodontal Ligament and Bone Marrow. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-463047
Modern Language Association (MLA)
Dong, Rui…[et al.]. Comparison of Long Noncoding RNA and mRNA Expression Profiles in Mesenchymal Stem Cells Derived from Human Periodontal Ligament and Bone Marrow. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-463047
American Medical Association (AMA)
Dong, Rui& Du, Juan& Wang, Liping& Wang, Jinsong& Ding, Gang& Wang, Songlin…[et al.]. Comparison of Long Noncoding RNA and mRNA Expression Profiles in Mesenchymal Stem Cells Derived from Human Periodontal Ligament and Bone Marrow. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-463047
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463047