Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352
Joint Authors
Ramachandra Murty, Vytla
Ramesh, Vinayagam
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Glucoamylase is an industrially important enzyme which converts soluble starch into glucose.
The media components for the production of glucoamylase from thermophilic fungus Humicola grisea MTCC 352 have been optimized.
Eight media components, namely, soluble starch, yeast extract, KH2PO4, K2HPO4, NaCl, CaCl2, MgSO4·7H2O, and Vogel’s trace elements solution, were first screened for their effect on the production of glucoamylase and only four components (soluble starch, yeast extract, K2HPO4, and MgSO4·7H2O) were identified as statistically significant using Plackett-Burman design.
It was fitted into a first-order model (R2=0.9859).
Steepest ascent method was performed to identify the location of optimum.
Central composite design was employed to determine the optimum values (soluble starch: 28.41 g/L, yeast extract: 9.61 g/L, K2HPO4: 2.42 g/L, and MgSO4·7H2O: 1.91 g/L).
The experimental activity of 12.27 U/mL obtained was close to the predicted activity of 12.15.
High R2 value (0.9397), low PRESS value (9.47), and AARD values (2.07%) indicate the accuracy of the proposed model.
The glucoamylase production was found to increase from 4.57 U/mL to 12.27 U/mL, a 2.68-fold enhancement, as compared to the unoptimized medium.
American Psychological Association (APA)
Ramesh, Vinayagam& Ramachandra Murty, Vytla. 2014. Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352. Enzyme Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-463053
Modern Language Association (MLA)
Ramesh, Vinayagam& Ramachandra Murty, Vytla. Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352. Enzyme Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-463053
American Medical Association (AMA)
Ramesh, Vinayagam& Ramachandra Murty, Vytla. Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352. Enzyme Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-463053
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463053